Colin Douglas.

colindougl.as/cv
me@colindougl.as
linkedin.com/in/colinddouglas

EDUCATION

University of Toronto, Toronto, ON
Ph.D., Biological and Organic Chemistry
Supervisor: Dr. Deborah Zamble
Thesis: Metal Specificity in [NiFe] Hydrogenase Maturation Proteins

St. Francis Xavier University, Antigonish, NS
B.Sc., Chemistry
Supervisor: Dr. Truis Smith-Palmer
Thesis: “Surface Enhanced Raman Spectroscopy of Microbiological Systems”

EXPERIENCE

Public Services and Procurement Canada (Ottawa, ON)
Senior Analyst (EC-06), February 2022 - Present
Data Scientist (EC-04), November 2020 - February 2022

I use R, a statistical programming language, to help people understand whether the policies they’re implementing are working.

Precision Biologic Inc. (Dartmouth, NS)
Research and Development Scientist
June 2017 - November 2020

I helped develop and clear two medical devices:

• CryoCheck Factor VIII Inhibitor Kit, which detects inhibitory factor VIII antibodies (K183440)
• CryoCheck Hex LA, which detects lupus anticoagulant through the neutralization of hexagonal phase phospholipid (K193556)

Faculty of Medicine, Dalhousie University (Halifax, NS)
Killam Postdoctoral Fellow
April 2015 - May 2017

I studied mechanism-based inhibitors of enolase enzymes. In one project, I used multivariate non- linear curve fitting to suss out the different steps in an inhibition mechanism, and demonstrated that one molecule we worked with was the tightest known inhibitor of it’s target enzyme.

POSTERS, PRESENTATIONS, AND PAPERS

• C.D. Douglas, L. Grandinetti, N.M. Easton, O.P. Kuehm, J.A. Hyaden, M.C. Hamilton, M. St. Maurice, S.L. Bearne. (2021). Slow-Onset, Potent Inhibition of Mandelate Racemase by 2-Formylphenylboronic Acid. An Unexpected Adduct Clasps the Catalytic Machinery. Biochemistry 60, 2508-18. [ft]
• C.D. Douglas, R. Clarke, N. Kesavan, D. Lamont, A. Sadeghi-Khomami, A. Wood, K.M. Black (Oct 2020). Comparison of Hexagonal Phase Phospholipid Neutralization Assays for Lupus Anticoagulant Detection. Poster presented at the THSNA 2020 Virtual Conference.
• C.D. Douglas, R. Clarke, N. Kesavan, D. Lamont, A. Sadeghi-Khomami, A. Wood, K.M. Black (July 2020). Laboratory Validation of a Novel Hexagonal Phase Phospholipid Neutralization Assay for Lupus Anticoagulant Detection. Poster presented at the ISTH Virtual Congress.
• C.D. Douglas, A. Wood, A. Sadeghi-Khomami, K.M. Black (September 2019). Effects of Common Interferents in Hexagonal Phase Phospholipid Neutralization Testing. Poster presented at the 16th International Congress on Antiphospholipid Antibodies, Manchester, UK.
• C.M. Fetter, Z.A. Morrison, M. Nagar, C.D. Douglas, S.L. Bearne (2019). Altering the Y137-K164-K166 triad of mandelate racemase and its effect on the observed pKa of the Brønsted base catalysts. Arch. Biochem. Biophys. 666, 116-126. [ft]
• C.D. Douglas, T. Quinton, E. Cabanban, K.M. Black, A. Sadeghi-Khomami (December 2018). A Standardized Kit for a Chromogenic Modified Nijmegen-Bethesda Assay. Poster presented at the 60th ASH Annual Meeting, San Diego, CA.
• C.D. Douglas, S.L. Bearne (June 2017). 2-Formylphenylboronate, a Potent Inhibitor of Mandelate Racemase. Oral presentation at the 100th Canadian Chemistry Conference and Exhibition, Toronto, ON.
• M.J. Lacasse, C.D. Douglas, D.B. Zamble (2016). Mechanism of Selective Nickel Transfer from Hydrogenase Accessory Proteins. Biochemistry 55, 6821–31. [ft]
• C.D. Douglas, D.B. Zamble (January 2014). The role of accessory proteins in the nickel insertion step of E. coli [NiFe] hydrogenase maturation. Oral presentation at the Gordon Conference on Metals in Biology, Ventura, CA.
• C.D. Douglas, T.T. Ngu, H. Kaluarachchi, D.B. Zamble (2013). Metal Transfer within the Escherichia coli HypB–HypA Complex of Hydrogenase Accessory Proteins. Biochemistry 52, 6030–6039. [ft]
• A.M. Sydor, M. Jost, K.S. Ryan, K.E. Turo, C.D. Douglas, C.L. Drennan, D.B. Zamble (2013). Metal binding properties of Escherichia coli YjiA, a member of the metal homeostasis-associated COG0523 family of GTPases. Biochemistry 52, 1788–1801. [ft]
• C.D. Douglas, A.D. Dias, D.B. Zamble (2012). The metal selectivity of a short peptide maquette imitating the high-affinity metal-binding site of E. coli HypB. Dalton Trans. 41, 7876–7878. [ft]
• C.D. Douglas, A.D. Dias, D.B. Zamble (June 2010). Probing the Selectivity of a High-Affinity Metal-Binding Peptide. Poster presentation at the 93rd Canadian Chemistry Conference and Exhibition, Toronto, ON.
• T. Smith-Palmer, C.D. Douglas, P. Fredericks (2010). Rationalizing the SER spectra of bacteria. Vib. Spectrosc. 53, 103–106. [ft]
• J.W.D. Comeau, J. Pink, E. Bezanson, C.D. Douglas, D. Pink, T. Smith-Palmer (2009). A comparison of Pseudomonas aeruginosa biofilm development on ZnSe and TiO2 using attenuated total reflection Fourier transform infrared spectroscopy. Appl. Spectrosc. 63, 1000–1007. [ft]
• M. Kazanci, J.P. Schulte, C.D. Douglas, P. Fratzl, D. Pink, T. Smith-Palmer (2009). Tuning the surface-enhanced Raman scattering effect to different molecular groups by switching the silver colloid solution pH. Appl. Spectrosc. 63, 214–223. [ft]
• D.T. Walker, C.D. Douglas, B.J. MacLean (2009). Synthesis, characterization, and surface studies of conjugated polymers possessing 2,2’-biimidazole moieties. Can. J. Chem. 87, 729–737. [ft]
• C.D. Douglas, D. Pink, T. Smith-Palmer (2009, June). Surface-enhanced Raman Scattering of Microbiological Systems. Poster presentation at the 92nd Canadian Chemistry Conference and Exhibition, Hamilton, ON.

AWARDS AND RECOGNITION

OPEN SOURCE CONTRIBUTIONS

TECHNICAL STRENGTHS

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